Table 1 Forward and reverse sequencing primers
Primer | Sequence range (bp) | Start site | 5′-Sequence-3′ |
|---|---|---|---|
FOR1 | 501–1000 | 448 | GACGGACCCAGAAGTGACC |
FOR2 | 1001–1500 | 948 | CCTTGGAAGTAGTAGATAAAGGATTCA |
FOR3 | 1501–2000 | 1453 | AGTGGTTCAGAGTTCCATCG |
FOR4 | 2001–2500 | 1948 | CAAAGTCTTGAGTTACCTCGG |
FOR5 | 2501–3000 | 2950 | TGTGTGAAGCAGGAGGAGTG |
REV1 | 500–1 | 552 | CTGATCGTGATGCCAGCTT |
REV2 | 1000–501 | 1040 | CAATCAGATCCACATTCTGTCC |
REV3 | 1500--1001 | 1542 | GCAGAACTCCTGCCTACATTG |
REV4 | 2000--1501 | 2040 | TATTCTGTAATGACCAAGGTGGG |
REV5 | 2500--2001 | 2552 | TGAAAATGCTCTCAGGGGC |